Anti-HA Tag Antibody

Product Details


Clone12CA5
ApplicationELISA, WB, Flow Cytometry, Sandwich ELISA
ReactivityHA tag, All Species Expected
FormatPurified
Target NameHA epitope tag, YPYDVPDYA tag, Hemagglutinin tag
IsotypeMouse IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Protein Concentration0.5 mg/mL
Storage&HandlingQuick spin the vial after receiving. The antibody solution should be stored between 2°C and 8°C without dilution.
Recommended UsageFor ELISA application, this antibody can be used at 1 ng/mL or lower as the detection antibody. For flow cytometric staining, it is recommended to use less than 0.2 ug of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. For detection, use a secondary reagent with this product.
See All FormatsClone 12CA5

Background Information


The HA tag (hemagglutinin tag) is a short linear epitope (YPYDVPDYA) derived from amino acids 98–106 of influenza A hemagglutinin. With a molecular weight of approximately 1.1 kDa, it is commonly fused to the N- or C-terminus of recombinant proteins to facilitate detection, localization, and purification when protein-specific antibodies are unavailable. Anti-HA tag antibodies specifically recognize the HA epitope and are widely used in applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and intracellular flow cytometry to study protein expression, topology, and protein–protein interactions.

Isotype Control


Mouse IgG2b Isotype Control Antibody

Data Sheets


Anti-HA Tag Antibody TDS

Related Protocols


Direct ELISA Protocol

Western Blot Protocol

Flow Cytometry Protocol

Sandwich ELISA with Streptavidin-Biotin Detection Protocol

Sandwich ELISA with Direct Detection Protocol

Related Products


Anti-His tag Antibody, Clone BR20M03

Biotin Anti-His tag Antibody, Clone BR20M03

Anti-c-Myc Antibody, Clone 9E10

Anti-DYKDDDDK Antibody, Clone BR20M05

Anti-Human TAG-72 (Minretumomab Biosimilar), Clone Minretumomab

iF647 Anti-His tag Antibody, Clone 1005AB

iF647 Anti-c-Myc Antibody, Clone 9E10

iF488 Anti-DYKDDDDK Antibody, Clone BR20M05

iF647 Anti-DYKDDDDK Antibody, Clone 1002AH1

iF488 Anti-His tag Antibody, Clone 1005AB

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.