Name: Anti-Human CD20 (Rituximab Biosimilar)
Brand: InnoCyto
SKU: 505301
Price: 190 USD
Availability: InStock

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Anti-Human CD20 (Rituximab Biosimilar)

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Cat #	Size	Price
505301	1 mg	$190
505302	5 mg	$590
505303	20 mg	$1800

Bulk/Custom

Product Details

Clone	Rituximab
Application	Flow cytometry, animal model study
Host Species	Mammalian cells
Reactivity	Human
Format	Liquid
Product Description	Rituximab Biosimilar, CD20 Monoclonal Antibody
Isotype	Human IgG1
Regulatory Status	RUO
Clonality	Recombinant
Immunogen	Human lymphoblastoid cell line SB
Clone Number	10F381
Species specificity	Human
Purity	>95% by reducing SDS-PAGE
Grade	In vivo
Min Sample Size	1 mg
Storage Conditions	4ºC
Maximal Shelf Life	12 months
RRID	AB_3739329
Target Name	CD20, MS4A-1
Antibody Type	Recombinant
Research Areas	B cells, Cancer Marker
See All Formats	Clone Rituximab

Background Information

Rituximab is a chimeric monoclonal antibody of the immunoglobulin G1 kappa (IgG1κ) subclass designed to target the CD20 antigen, a transmembrane phosphoprotein expressed on the surface of B lymphocytes. Structurally, it is a glycoprotein with a molecular weight of approximately 145 kilodaltons (kDa) and consists of two identical heavy chains and two identical light chains connected by disulfide bonds, forming the Y-shaped quaternary configuration typical of IgG molecules. The heavy and light chains each contain variable (VH and VL) and constant (CH1–CH3 and CL) domains, with the variable regions defining the antibody’s antigen-binding specificity. Rituximab is produced through recombinant DNA technology using mammalian expression systems, such as Chinese Hamster Ovary (CHO) cells, ensuring proper folding, glycosylation, and structural stability.

The antigen-binding fragments (Fab) of Rituximab contain complementarity-determining regions (CDRs) derived from murine antibody sequences that confer high specificity for a conformational epitope on the large extracellular loop of CD20. The constant domains are human IgG1 sequences, reducing immunogenicity and enhancing serum stability. Rituximab associates tightly with CD20, which acts as an integral component of calcium flux regulation and B-cell activation in experimental models. The engagement of CD20 by Rituximab can induce conformational changes and aggregation in lipid rafts, leading to intracellular signaling events that include calcium mobilization, phosphorylation of kinases, and sometimes direct apoptotic signaling in target cells.

The Fc (fragment crystallizable) portion of Rituximab contributes to effector functions characteristic of the human IgG1 subclass. It interacts with Fc gamma receptors (FcγRs) on immune effector cells to mediate antibody-dependent cellular cytotoxicity (ADCC) and with complement component C1q to trigger complement-dependent cytotoxicity (CDC) in vitro. The Fc segment also binds to the neonatal Fc receptor (FcRn), enabling molecular recycling and prolonged half-life. Overall, Rituximab exemplifies rational antibody engineering—integrating specific antigen recognition, structural integrity, and effector functionality—to study B-cell biology and Fc-mediated immune mechanisms.

Frequently Asked Questions

What are InnoCyto Biosimilar products?

InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.

How similar are these biosimilars to the original therapeutic antibody?

Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.

Are these antibodies suitable for in vivo studies?

Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.

What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?

A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.

What quality and purity standards do biosimilar antibodies meet?

All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.

Which therapeutic targets and mechanisms are covered in the Biosimilar line?

The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

Have a product or application question? Consult our FAQs or contact us.

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