Anti-Human CD57 Antibody

Cat # Size Price Quantity
110501500 ug$220

Product Details


CloneHNK-1
ApplicationFlow Cytometry
ReactivityHuman
FormatPurified
Target NameCD57, HNK-1, Leu-7, NK-1
IsotypeMouse IgM
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Protein Concentration0.5 mg/mL
Storage&HandlingThe antibody solution should be stored between 2°C and 8°C
Recommended UsageFor flow cytometric staining, it is recommended to use less than 0.2 µg of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
RRIDAB_3738942
Research AreasNK cells, T cells, Myelin, Neurons, Cancer Marker, CMV
See All FormatsClone HNK-1

Background Information


CD57 (HNK-1, NK-1, Leu-7) is a 100–115 kDa oligosaccharide antigen expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. It is found on terminally differentiated NK cells, CD8⁺ T cells, and a subset of peripheral blood lymphocytes, as well as on neural cells and striated muscle, but not on red blood cells, granulocytes, monocytes, or platelets. Although its precise function is unclear, CD57 can bind L-selectin, P-selectin, and laminin fragments, suggesting a role in cell–matrix interactions. CD57⁺ immune cells exhibit potent cytotoxicity but reduced proliferation and cytokine responsiveness. Clinically, CD57 expression is elevated in conditions with CD4/CD8 imbalances, including chronic viral infections, autoimmune diseases, AIDS, and post-transplant states, while low levels may be observed in conditions like Lyme disease.

Isotype Control


Mouse IgM Isotype Control Antibody

Data Sheets


Anti-Human CD57 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


Anti-Human CD56 (NCAM) Antibody, Clone 056EM1

iF647 Anti-Human CD56 (NCAM) Antibody, Clone 056EM1

In Vivo Star Anti-Human CD56 (NCAM) Antibody, Clone N901

iF488 Anti-Human CD56 Antibody, Clone CD56H

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.