Anti-Human IL-2 Antibody

Product Details


CloneMQ1-17H12
ApplicationICFC, ELISA, Sandwich ELISA
ReactivityHuman
FormatPurified
Target NameIL-2, Interleukin-2, T cell growth factor,Macrophage-activating factor for cytotoxicity I, Killer cell helper factor
IsotypeRat IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Protein Concentration0.5 mg/mL
Storage&HandlingThe antibody solution should be stored between 2°C and 8°C
Recommended UsageFor flow cytometric staining, it is recommended to use less than 0.2 ug of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application
Research AreasCytokines, Inflammation, Autoimmunity, T cells, Tregs, NK cells, B cells, Cancer Immunology
See All FormatsClone MQ1-17H12

Background Information


Interleukin-2 (IL-2) is a potent immunomodulatory cytokine primarily produced by activated CD4⁺ T helper cells. It plays a central role in immune activation and tolerance by promoting the proliferation and differentiation of T cells, NK cells, and B cells. IL-2 signals through a heterotrimeric IL-2 receptor complex composed of α (CD25), β, and common γ chains, activating downstream JAK/STAT, PI3K, and MAPK pathways. IL-2 is essential for regulatory T cell development and maintenance, thereby preventing autoimmunity. Human IL-2 shares significant sequence homology with mouse IL-2 and exhibits cross-species activity, supporting its broad application in immunological research and cell culture–based assays

Isotype Control


Rat IgG2a Isotype Control Antibody

Data Sheets


Anti-Human IL-2 Antibody TDS

Related Protocols


Direct ELISA Protocol

Sandwich ELISA with Streptavidin-Biotin Detection Protocol

Sandwich ELISA with Direct Detection Protocol

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Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.