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Anti-Human IL-6R (Tocilizumab Biosimilar)

Cat # Size Price Quantity
5060011 mg$190
5060025 mg$590
50600320 mg$1800

  Bulk/Custom

Product Details

CloneTocilizumab
ApplicationFlow cytometry, animal model study
Host SpeciesMammalian cells
ReactivityHuman
FormatLiquid
Product DescriptionTocilizumab Biosimilar, Human IL-6R Monoclonal Antibody
IsotypeHuman IgG1
Regulatory StatusRUO
ClonalityRecombinant
ImmunogenHuman IL-6R
Species specificityHuman
Purity>95% by reducing SDS-PAGE
GradeIn vivo
Storage Conditions4ºC
Maximal Shelf Life12 months
SynonymsCD126, gp80
Target NameIL-6R, IL-6R1, gp80, IL-6 receptor alpha
Antibody TypeRecombinant
Research AreasB cells, T cells, Autoimmunity, Cancer Marker
See All FormatsClone Tocilizumab

Background Information

Tocilizumab is a humanized monoclonal antibody belonging to the immunoglobulin G1 kappa (IgG1κ) subclass, engineered to specifically bind to the interleukin-6 receptor (IL-6R), both its soluble (sIL-6R) and membrane-bound (mIL-6R) forms. Structurally, Tocilizumab is a glycoprotein with a molecular mass of approximately 148 kilodaltons (kDa). It comprises two identical heavy chains and two identical light chains connected by interchain disulfide bonds, forming the classical Y-shaped IgG quaternary structure. Each heavy chain contains one variable (VH) and three constant (CH1-CH3) domains, while each light chain is composed of one variable (VL) and one constant (CL) domain. The antibody is expressed in mammalian cell systems such as Chinese Hamster Ovary (CHO) cells, which enable correct folding, glycosylation, and assembly.

The variable regions of Tocilizumab, particularly the complementarity-determining regions (CDRs), are derived from murine antibody sequences and engineered into a human IgG1 framework. These CDRs form the antigen-binding sites that confer high affinity and selectivity toward IL-6R. Tocilizumab binds to an epitope on the extracellular domain of IL-6R, preventing interleukin-6 (IL-6) from interacting with its receptor complex. This steric blockade inhibits the formation of the IL-6/IL-6R/gp130 signaling complex, which is responsible for activating intracellular pathways such as JAK/STAT, MAPK, and PI3K/AKT. Consequently, the antibody modulates cytokine-mediated transcriptional regulation, cell survival, and immune signaling in model systems.

The Fc (fragment crystallizable) region of Tocilizumab, derived from the human IgG1 isotype, contributes to structural stability and prolonged circulation via neonatal Fc receptor (FcRn)-mediated recycling. The Fc region also allows limited engagement with Fc gamma receptors (FcγRs), though the primary mechanism of action is ligand-receptor blockade rather than effector-mediated cytotoxic activity.

Data Sheets

Anti-Human IL-6R (Tocilizumab Biosimilar) TDS

Related Protocols

Flow Cytometry Protocol

Frequently Asked Questions

What are InnoCyto Biosimilar products?
InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.
How similar are these biosimilars to the original therapeutic antibody?
Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.
Are these antibodies suitable for in vivo studies?
Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.
What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?
A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.
What quality and purity standards do biosimilar antibodies meet?
All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.
Which therapeutic targets and mechanisms are covered in the Biosimilar line?
The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

Have a product or application question? Consult our FAQs or contact us.