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Anti-Human integrin α4β1 (VLA-4)

Cat # Size Price Quantity
5040011 mg$175
5040025 mg$480
50400320 mg$1390

  Bulk/Custom

Product Details

CloneNatalizumab
ApplicationFlow cytometry, animal model study
Host SpeciesMammalian cells
ReactivityHuman
FormatLiquid
Product DescriptionNatalizumab Biosimilar, Integrin alpha 4 Monoclonal Antibody
IsotypeHuman IgG4
Regulatory StatusRUO
ClonalityRecombinant
ImmunogenHuman Integrin alpha 4
Species specificityHuman
Purity>95% by reducing SDS-PAGE
GradeIn vivo
Storage Conditions4ºC
Maximal Shelf Life12 months
SynonymsAlpha 4 integrin, a4 integrin
RRIDAB_3739316
Target NameVLA-4 α chain, α4 integrin, integrin α4, ITGA4
Antibody TypeRecombinant
Research AreasAdhesion Molecules, T cells, B cells, NK cells, Hematopoietic Stem Cells, Autoimmunity, Cancer Marker
See All FormatsClone Natalizumab

Background Information

Natalizumab is a recombinant humanized monoclonal antibody belonging to the immunoglobulin G4 (IgG4) subclass, designed to target the α4 subunit of integrins expressed on the surface of leukocytes. Structurally, the molecule has a molecular weight of approximately 149 kilodaltons (kDa) and is composed of two identical heavy chains and two identical light chains joined by interchain disulfide bonds, forming the classical Y-shaped IgG conformation. The heavy chains each contain one variable (VH) domain and three constant (CH1-CH3) domains, while the light chains consist of one variable (VL) and one constant (CL) domain. Natalizumab is produced in mammalian expression systems such as Chinese Hamster Ovary (CHO) cells to ensure proper protein folding, assembly, and glycosylation.

The antigen-binding sites of Natalizumab reside in the complementarity-determining regions (CDRs) within its VH and VL domains, which confer high specificity toward the α4 integrin subunit (also known as CD49d). This subunit forms heterodimers with β1 or β7 integrin partners to generate α4β1 (very late antigen-4, VLA-4) and α4β7 integral membrane receptors. By binding to α4 integrins, Natalizumab sterically inhibits their interaction with endothelial ligands, such as vascular cell adhesion molecule-1 (VCAM-1) and mucosal addressin cell adhesion molecule-1 (MAdCAM-1). In experimental systems, this blockade interferes with leukocyte adhesion, migration, and trafficking through endothelial barriers, thereby modulating immune cell localization and activity within tissues.

The Fc region of Natalizumab is derived from the human IgG4 isotype, which is characterized by a naturally reduced capacity to engage effector mechanisms such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). This subclass also incorporates a stabilizing S228P substitution to prevent half-antibody exchange, enhancing molecular integrity. Overall, Natalizumab exemplifies a rationally engineered IgG4 monoclonal antibody combining high-affinity integrin blockade with structural stability to precisely modulate cell adhesion and immune signaling mechanisms in molecular and cellular research contexts.

Data Sheets

Anti-Human integrin α4β1 (VLA-4) TDS

Related Protocols

Flow Cytometry Protocol

Frequently Asked Questions

What are InnoCyto Biosimilar products?
InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.
How similar are these biosimilars to the original therapeutic antibody?
Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.
Are these antibodies suitable for in vivo studies?
Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.
What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?
A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.
What quality and purity standards do biosimilar antibodies meet?
All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.
Which therapeutic targets and mechanisms are covered in the Biosimilar line?
The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

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