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Anti-Human TNFα (Adalimumab Biosimilar)

Cat # Size Price Quantity
5003011 mg$190
5003025 mg$590
50030320 mg$1800

  Bulk/Custom

Product Details

CloneAdalimumab
ApplicationNeutralization, Intracellular Flow cytometry, animal model study
Host SpeciesMammalian cells
ReactivityHuman
FormatLiquid
Product DescriptionAnti-Human TNFα (Adalimumab Biosimilar)
IsotypeHuman IgG1
Regulatory StatusRUO
ClonalityRecombinant
ImmunogenHuman TNF alpha
Clone NumberD2E7
Species specificityHuman
Purity>95% by reducing SDS-PAGE
GradeIn vivo
Min Sample Size1 mg
Storage Conditions4ºC
Maximal Shelf Life12 months
SynonymsTNFa, TNF-alpha
RRIDAB_3739279
Target NameTNFα
Research AreasCytokines, Monocytes, Macrophages, Neutrophils, T cells, Inflammation, Autoimmunity
See All FormatsClone Adalimumab

Background Information

Adalimumab is a fully human monoclonal antibody that belongs to the immunoglobulin G1 (IgG1) subclass. It was developed using recombinant DNA technology in mammalian cell expression systems, such as Chinese Hamster Ovary (CHO) cells, to ensure proper protein folding, glycosylation, and functional integrity. Structurally, Adalimumab is a 148-kilodalton (kDa) glycoprotein composed of two identical heavy chains and two identical light chains connected by disulfide bonds, resulting in a typical Y-shaped antibody configuration. Each heavy chain consists of variable and constant regions, including CH1, CH2, and CH3 domains, while each light chain contains one variable and one constant domain. The molecule contains N-linked glycosylation sites in the Fc region, which contribute to stability, solubility, and interactions with immune effectors.

The functional activity of Adalimumab derives from its high-affinity binding to tumor necrosis factor-alpha (TNF-α), a proinflammatory cytokine involved in immune signaling and regulation. Each Fab fragment of Adalimumab contains complementarity-determining regions (CDRs) that specifically recognize and bind to soluble and transmembrane forms of human TNF-α with nanomolar affinity. By binding tightly to TNF-α, the antibody prevents the cytokine from interacting with its cellular receptors, TNFR1 (p55) and TNFR2 (p75), effectively blocking downstream signaling pathways that lead to the expression of inflammatory mediators, adhesion molecules, and other immune-regulatory proteins.

In addition to its antigen-binding capacity, Adalimumab’s Fc region mediates secondary molecular interactions important for immune modulation. These include binding to neonatal Fc receptors (FcRn), which extend the antibody’s circulatory half-life by protecting it from lysosomal degradation, and engagement with Fc gamma receptors (FcγRs), though Adalimumab is engineered to minimize undesired effector functions such as complement activation. Overall, Adalimumab represents a well-characterized biologic molecule whose precise molecular architecture enables high target specificity, structural stability, and controlled immunomodulatory function in research applications studying cytokine signaling and immune regulation.

Data Sheets

Anti-Human TNFα (Adalimumab Biosimilar) TDS

Related Products

Anti-Human TNF alpha (Infliximab Biosimilar), Clone Infliximab

Frequently Asked Questions

What are InnoCyto Biosimilar products?
InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.
How similar are these biosimilars to the original therapeutic antibody?
Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.
Are these antibodies suitable for in vivo studies?
Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.
What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?
A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.
What quality and purity standards do biosimilar antibodies meet?
All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.
Which therapeutic targets and mechanisms are covered in the Biosimilar line?
The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

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