| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 206801 | 25 µg | $30 | ||
| 206802 | 100 µg | $60 |
| Clone | YN1/1.7.4 |
|---|---|
| Application | Flow Cytometry, IHC-F, IP, WB |
| Reactivity | Mouse |
| Format | Purified |
| Target Name | CD54, ICAM-1, Ly-47 |
| Isotype | Rat IgG2b |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Protein Concentration | 0.5 mg/mL |
| Storage&Handling | The antibody solution should be stored between 2°C and 8°C |
| See All Formats | Clone YN1/1.7.4 |
Mouse CD54, also known as intercellular adhesion molecule-1 (ICAM-1), is a transmembrane glycoprotein encoded by the Icam1 gene and is a member of the immunoglobulin superfamily. It is constitutively expressed at low levels on endothelial cells, epithelial cells, fibroblasts, and many leukocytes, but is strongly upregulated by inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interferon-γ (IFN-γ). CD54 plays a central role in immune surveillance by mediating leukocyte adhesion to vascular endothelium and promoting migration of immune cells into inflamed tissues.
Structurally, mouse CD54 consists of five extracellular immunoglobulin-like domains, a single transmembrane domain, and a short cytoplasmic tail. Although the cytoplasmic domain lacks intrinsic enzymatic activity, it participates in intracellular signaling through interactions with cytoskeletal and adaptor proteins. The principal ligands for CD54 are the β2 integrins lymphocyte function-associated antigen-1 (LFA-1; CD11a/CD18) and macrophage-1 antigen (Mac-1; CD11b/CD18), which are expressed on leukocytes. These interactions stabilize immune cell adhesion, facilitate antigen presentation, and support T cell activation.
CD54 has been implicated in numerous inflammatory and autoimmune diseases in mouse models, including experimental autoimmune encephalomyelitis, rheumatoid arthritis, inflammatory bowel disease, and transplant rejection. Elevated CD54 expression also contributes to leukocyte infiltration during infection and tissue injury. Conversely, reduced CD54 function can impair effective immune responses. In cancer, CD54 expression may either enhance antitumor immunity by promoting immune cell interactions or facilitate tumor progression depending on the tumor microenvironment.
Because of its central role in leukocyte trafficking and immune activation, CD54 is an attractive therapeutic target. Blocking CD54 or its interaction with LFA-1 can reduce excessive inflammation in preclinical models, while strategies that increase CD54 expression on tumors may improve immune-mediated tumor killing. Consequently, mouse CD54 is widely used in studies investigating inflammatory disease mechanisms and the development of immunomodulatory therapies.
Anti-Mouse CD54 (ICAM-1) Antibody TDS
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