NK-1.1, also known as CD161b/CD161c or Ly-55, is a type II transmembrane C-type lectin receptor encoded by the Klrb1 (NKR-P1) gene family in mice. It is predominantly expressed on natural killer (NK) cells and a subset of invariant NKT cells in selected mouse strains, including C57BL/6, FVB/N, and NZB. The activating isoform NKR-P1C lacks an inhibitory motif and promotes NK cell cytotoxicity, IFN-γ production, and tumor cell lysis. NK-1.1 expression correlates with effective immune surveillance and graft rejection, making it a widely used marker for identifying and studying murine NK and NKT cell populations.
How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.
Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.
What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.
Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.
Have a product or application question? Consult our FAQs or contact us.
