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Biotin Annexin V

Cat # Size Price Quantity
60420325 tests$70
604204100 tests$170

  Bulk/Custom

Product Details

ApplicationFlow Cytometry
FormatBiotin
Expression HostE.coli
Target NameAnnexin A5
SpeciesHuman
accession numberP08758
SourcesRecombinant Human Annexin A5 (Met1-Asp320) with N-His-Xa tag is expressed in E.coli system. His tag is cut by Xa after purification. This protein is biotinylated in vitro.
Molecular WeightThis protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE.
Affinity TagNone
Purity>95% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Protein ConcentrationSupplied at a lot-specific concentration.
Storage and HandlingThe protein solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Research AreasApoptosis, Cell Death

Background Information

Annexin A5 is a member of the annexin family, a group of proteins that bind negatively charged phospholipids in a calcium-dependent manner. During apoptosis, phosphatidylserine (PS) translocates to the outer leaflet of the plasma membrane. Annexin A5 specifically binds to this exposed PS, making it a widely used marker for early apoptosis. It is commonly used in combination with propidium iodide (PI) in flow cytometry or microscopy assays

Data Sheets

Biotin Annexin V TDS

Related Protocols

Flow Cytometry Protocol

Frequently Asked Questions

What is Annexin V, and what is it used for?
Annexin V is a calcium-dependent phospholipid-binding protein that binds with high affinity to phosphatidylserine (PS), a membrane phospholipid that flips from the inner to the outer leaflet of the plasma membrane early in apoptosis. This makes Annexin V a standard reagent for detecting and quantifying apoptotic cells by flow cytometry, fluorescence microscopy, or imaging.
Why do I need calcium (Ca²⁺) in my binding buffer when using Annexin V?
Annexin V binding to phosphatidylserine is strictly calcium-dependent, so a Ca²⁺-containing binding buffer is required for proper staining — using a calcium-free buffer (e.g., standard PBS) will result in little to no signal. Avoid EDTA/EGTA-containing buffers during staining.
What controls should I include in an Annexin V apoptosis assay?
Recommended controls include unstained cells (baseline autofluorescence), single-stain controls for Annexin V and any viability dye (for compensation), and a known apoptosis-induced positive control sample (e.g., treated with a standard apoptosis-inducing agent) to confirm assay sensitivity. Untreated/healthy cells also serve as a useful negative control for background PS exposure.

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