FITC Rat IgG2a Isotype Control Antibody

Product Details


CloneRTK2758
ApplicationFlow Cytometry
ReactivityN/A
FormatFITC
Target NameRat IgG2a Isotype control
IsotypeRat IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration0.2mg/mL
Storage and HandlingStore the antibody undiluted at 2°C to 8°C.
Recommended UsageUse at concentrations comparable to those used for the target-specific antibody. FITC has an excitation max at 493 nm and an emission max at 525 nm.
Excitation LaserBlue Laser (488 nm)
RRIDAB_3739123
See All FormatsClone RTK2758

Background Information


The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Data Sheets


FITC Rat IgG2a Isotype Control Antibody TDS

Related Protocols


Flow Cytometry Protocol

Frequently Asked Questions


What should I do if I don't see an isotype control available for my antibody of choice?
Contact Technical Support or request a quote for a custom conjugation of the appropriate isotype and fluorophore.

Why should I use an isotype control?
Isotype controls are antibodies with no known specificity for the target of interest, but matched in host species, isotype, and conjugate to your primary antibody. They are used to measure non-specific binding (e.g., Fc receptor binding, background staining) so that signal from your specific antibody can be distinguished from background noise.

What should I do if my isotype control shows high background binding?
High background is often observed due to a number of causes such as Fc binding, poor cell viability, spillover and unmixing error, as well as monocyte tandem binding. Read this brief tech note to learn about the causes and specific solutions to remove background signals: The Path to True Signals: Removing Background in Flow Cytometry.

Have a product or application question? Consult our FAQs or contact us.