FLAER

Product Details


ApplicationFlow Cytometry
FormatLiquid, iFlour 488
Expression HostE.coli
Target NamePro-aerolysin, FLAER, aerolysin, AER
SpeciesAeromonas hydrophila
SourcesRecombinant Pro-aerolysin (Ala24-Arg453) with N-Met and C-terminus His tag is expressed in E.coli system. This protein is conjugated to iFlour 488.
Molecular WeightThis protein has a predicted molecular weight of 53 kDa. Under DTT-reducing conditions, the protein migrates at approximately 55 kDa on SDS-PAGE prior to conjugation.
Affinity TagC-His
Purity>95% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation1xPBS/0.05%NaN3/0.2%BSA
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.3mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Research AreasParoxysmal Nocturnal Hemoglobinuria

Background Information


Pro-aerolysin is the inactive precursor of aerolysin, a pore-forming toxin produced by the bacterium Aeromonas hydrophila. It has been engineered to be non-toxic. FLAER is a fluorescently labeled derivative of pro-aerolysin and serves as a valuable reagent for detecting cells that express GPI-anchored proteins.

Data Sheets


FLAER TDS

Related Protocols


Flow Cytometry Protocol

Frequently Asked Questions


Why are Recombinant Proteins expressed with epitope tags?
The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.

What types of tagged proteins are best for my application?
Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.

Does the tag affect the protein's biological activity or binding function?
Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.