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GST Protein with multiple tags

Cat # Size Price Quantity
60390125 ug$79
603902100 ug$199
603903500 ug$599
6039041 mg$749

  Bulk/Custom

Product Details

ApplicationELISA, BLI
FormatLiquid, Purified
Expression HostE.coli
Target NameGST with multiple tags protein (Myc-Avi-V5-DYKDDDDK-HA-His)
SpeciesSchistosoma japonicum
SourcesRecombinant GST protein with multiple tags (Myc-Avi-V5-FLAG-HA-His) at C-terminus is expressed in E.coli system.
Molecular WeightThis protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE. The smaller band below 30 kDa might correspond to the early translational termination product.
Affinity TagC-Myc-Avi-V5-DYKDDDDK-HA-His
Purity>90% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.

Data Sheets

GST Protein with multiple tags TDS

Related Protocols

Direct ELISA Protocol

Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?
The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.
What types of tagged proteins are best for my application?
Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.
Does the tag affect the protein's biological activity or binding function?
Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.