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Human Alpha 1 Antitrypsin protein (Active)

Cat # Size Price Quantity
606701100 μg$450
6067021 mg$2500

  Bulk/Custom

Product Details

ApplicationBioassay
FormatLyophilized from sterile PBS, pH 7.4.
Expression HostHEK293 Cells
Target NameSerpin A1, A1AT, AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, , alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor
SpeciesHuman
accession numberNP_000286.3
SourcesRecombinant Human SerpinA1 (NP_000286.3) pre-protein (Met 1-Lys 418) with a C-terminal polyhistidine tag was expressed in HEK293 cells.
Molecular WeightThis protein has a calculated molecular mass of 45.7 kDa. Due to glycosylation, the rhSerpinA1 migrates as an approximately 55-60 kDa protein in SDS-PAGE analysis under reducing conditions.
Affinity TagC-His
Purity>95% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Endotoxin level< 1.0 EU per μg of the protein as determined by the LAL method
Protein ConcentrationLyophilized
Storage and HandlingSamples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Research AreasEmphysema, COPD

Background Information

Alpha-1 antitrypsin (A1AT), also known as SERPINA1 or alpha-1 protease inhibitor, is a major circulating serine protease inhibitor predominantly produced by hepatocytes and secreted into the bloodstream. A1AT plays a critical role in protecting tissues from proteolytic damage by irreversibly inhibiting a broad range of proteases, particularly neutrophil elastase. Dysregulation or genetic deficiency of A1AT is associated with the development of pulmonary emphysema, chronic obstructive pulmonary disease (COPD), liver disease, and inflammatory disorders.
In addition to its well-characterized anti-protease activity, A1AT has been reported to exhibit anti-inflammatory and immunomodulatory functions, making it an important target in respiratory disease research, inflammation biology, tissue injury studies, and therapeutic development. Recombinant Human Alpha 1 Antitrypsin protein is widely used in enzyme inhibition assays, cell culture applications, drug screening, protein interaction studies, and biomarker research.

Data Sheets

Human Alpha 1 Antitrypsin protein (Active) TDS

Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?
The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.
What types of tagged proteins are best for my application?
Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.
Does the tag affect the protein's biological activity or binding function?
Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.