| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 700601 | 100 Tests | $150 | ||
| 700602 | 500 Tests | $350 |
| Clone | N/A |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Human |
| Format | Purified |
| Target Name | Fc Blocker, FcγR blocker, Fc inhibitor |
| Isotype | Human IgG |
| Antibody Type | Recombinant |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Protein Concentration | 1.0 mg/mL |
| Storage&Handling | The antibody solution should be stored between 2°C and 8°C |
| Recommended Usage | For flow cytometric analysis, block Fc receptors by pre-incubating cells with a human Fc blocker for 5–10 minutes on ice at 2.5 µL per 10⁶ cells in a volume of 100 µL prior to immunostaining. Washing between the blocking step and primary antibody staining is not required. |
| See All Formats | Clone N/A |
Human Fc receptors (FcRs) are cell surface proteins that bind the Fc region of antibodies and mediate key immune functions such as phagocytosis, antibody-dependent cellular cytotoxicity, and cytokine release. They are widely expressed on immune cells, including monocytes, macrophages, dendritic cells, neutrophils, NK cells, and B cells.
In flow cytometry, Fc receptors can bind staining antibodies non-specifically through their Fc regions, leading to increased background and potential false-positive signals. To minimize this, Fc receptor blocking reagents are typically used prior to staining. This step is especially important when working with myeloid populations or other Fc receptor–rich cells, as it improves staining specificity and overall data accuracy.
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