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Human GM2A Protein (N-His)

Cat # Size Price Quantity
60150125 ug$145
601502100 ug$295

  Bulk/Custom

Product Details

ApplicationELISA, BLI
FormatLiquid, Purified
Expression HostHEK293
Target NameGM2A, Ganglioside GM2 activator
SpeciesHuman
Accession NumberP17900
SourcesRecombinant human GM2A mature fragment (Ser32-Ile193) with N-terminus His tag was expressed in 293 Cells
Molecular WeightThis protein has a predicted molecular weight of 19.7 kDa. Under DTT-reducing conditions, the protein migrates at approximately 22 kDa on SDS-PAGE.
Affinity TagN-His
Purity>95% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation1xPBS with 300mM NaCl, pH7.4, 0.22 µm filtered
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Research AreasMetabolism

Background Information

GM2 ganglioside activator (GM2A) is a lipid transfer protein of the ML domain family that facilitates the degradation of ganglioside GM2 by acting as a substrate-specific cofactor for β-hexosaminidase A. GM2A extracts GM2 from membranes and presents it in soluble form to β-hexosaminidase A for cleavage, enabling conversion to GM3. It can bind several single-chain phospholipids and fatty acids and shows some calcium-independent phospholipase activity. Mutations in GM2A cause GM2-gangliosidosis type AB, a Tay-Sachs disease variant characterized by impaired GM2 degradation and lipid accumulation.

Data Sheets

Human GM2A Protein (N-His) TDS

Related Protocols

Direct ELISA Protocol

Related Products

Human GM2A Protein (C-His)

Human GM2A Protein (C-FLAG)

Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?
The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.
What types of tagged proteins are best for my application?
Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.
Does the tag affect the protein's biological activity or binding function?
Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.