Name: Human HexB Protein (C-His)
Brand: InnoCyto
SKU: 603301
Price: 145 USD
Availability: InStock

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Human HexB Protein (C-His)

Purified HexB (C-His) final product on SDS-PAGE under non-reducing (P-) and reducing (P+) conditions. The purity of HexB appears to be greater than 95%.

Cat #	Size	Price	Quantity
603301	25 ug	$145
603302	100 ug	$295

Bulk/Custom

Product Details

Application	ELISA
Format	Liquid, Purified
Expression Host	HEK293
Target Name	Hexosaminidase B
Species	Human
Accession Number	P07686
Sources	Human HexB (Ala43-Met556) protein with C-terminus His tag is expressed in HEK293 cells.
Molecular Weight	This protein has a predicted molecular weight of 60.1 kDa. Under DTT-reducing conditions, the protein migrates at approximately 65 kDa on SDS-PAGE.
Affinity Tag	C-His
Purity	>95% based on SDS-PAGE under reducing condition
Regulatory Status	RUO
Formulation	25 mM Tris, 150 mM NaCl, pH 7.5 (0.2 μm filtered)
Endotoxin level	Not tested
Protein Concentration	25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling	Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered 25 mM Tris, 150 mM NaCl, pH 7.5. For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Research Areas	Metabolism

Background Information

Beta-hexosaminidases are lysosomal enzymes that hydrolyze terminal N-acetyl-D-hexosamine residues from GM2 gangliosides and globo-sphingolipids. They exist in three isoforms: Hex A (αβ), Hex B (ββ), and Hex S (αα), formed by different combinations of α and β subunits encoded by the HEXA and HEXB genes. Recombinant HexB corresponds to Hex B isoform.

Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?

The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.

What types of tagged proteins are best for my application?

Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.

Does the tag affect the protein's biological activity or binding function?

Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.

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