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Human IgM-Fc Protein

Cat # Size Price Quantity
60030125 ug$145
600302100 ug$295

  Bulk/Custom

Product Details

ApplicationELISA
FormatLiquid, Purified
Expression HostHEK293
Target NameHuman IgM-Fc protein
SpeciesHuman
Accession NumberP01871
SourcesHuman IgM-Fc (Val105-Gly434) protein with C-terminus His tag is expressed in HEK293 cells.
Molecular WeightThis protein has a predicted molecular weight of 37.8 kDa. Under DTT-reducing conditions, the protein migrates at approximately 40-45 kDa on SDS-PAGE.
Affinity TagNone
Purity>95% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.

Background Information

Fc fragments consist of the carboxy-terminal halves of both immunoglobulin heavy chains connected by disulfide bonds, forming the constant region responsible for antibody effector functions. These functions include complement fixation, binding to Fc receptors on immune cells (such as CD16, CD32, and CD64), and mediating placental transport of antibodies. The Fc region plays a critical role in immune defense by triggering processes like opsonization, antibody-dependent cellular cytotoxicity (ADCC), and inflammation. Additionally, proteins like Protein A and Protein G, found on certain bacteria, specifically bind to the Fc region and are widely used in biotechnology for antibody purification. Recombinant Fc fragments also have potential therapeutic applications as anti-inflammatory agents in autoimmune diseases.

Data Sheets

Human IgM-Fc Protein TDS

Related Protocols

Direct ELISA Protocol

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Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?
The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.
What types of tagged proteins are best for my application?
Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.
Does the tag affect the protein's biological activity or binding function?
Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.