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iF488 Annexin V

Cat # Size Price Quantity
60420925 tests$85
604210100 tests$190

  Bulk/Custom

Product Details

ApplicationFlow Cytometry
FormatiFlour 488
Expression HostE.coli
Target NameAnnexin A5
SpeciesHuman
accession numberP08758
SourcesRecombinant Human Annexin A5 (Met1-Asp320) with N-His-Xa tag is expressed in E.coli system. His tag is cut by Xa after purification. This protein is then conjugated to iF488.
Molecular WeightThis protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE prior to conjugation.
Affinity TagNone
Regulatory StatusRUO
Formulation1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Protein ConcentrationSupplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF488 has an excitation max at 491 nm and an emission max at 516 nm
Excitation LaserBlue Laser (488 nm)
Research AreasApoptosis, Cell Death

Background Information

Annexin A5 is a member of the annexin family, a group of proteins that bind negatively charged phospholipids in a calcium-dependent manner. During apoptosis, phosphatidylserine (PS) translocates to the outer leaflet of the plasma membrane. Annexin A5 specifically binds to this exposed PS, making it a widely used marker for early apoptosis. It is commonly used in combination with propidium iodide (PI) in flow cytometry or microscopy assays

Data Sheets

iF488 Annexin V TDS

Related Protocols

Flow Cytometry Protocol

Frequently Asked Questions

What is Annexin V, and what is it used for?
Annexin V is a calcium-dependent phospholipid-binding protein that binds with high affinity to phosphatidylserine (PS), a membrane phospholipid that flips from the inner to the outer leaflet of the plasma membrane early in apoptosis. This makes Annexin V a standard reagent for detecting and quantifying apoptotic cells by flow cytometry, fluorescence microscopy, or imaging.
Why do I need calcium (Ca²⁺) in my binding buffer when using Annexin V?
Annexin V binding to phosphatidylserine is strictly calcium-dependent, so a Ca²⁺-containing binding buffer is required for proper staining — using a calcium-free buffer (e.g., standard PBS) will result in little to no signal. Avoid EDTA/EGTA-containing buffers during staining.
What controls should I include in an Annexin V apoptosis assay?
Recommended controls include unstained cells (baseline autofluorescence), single-stain controls for Annexin V and any viability dye (for compensation), and a known apoptosis-induced positive control sample (e.g., treated with a standard apoptosis-inducing agent) to confirm assay sensitivity. Untreated/healthy cells also serve as a useful negative control for background PS exposure.

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