iF488 Anti-Human CD34 Antibody

Cat # Size Price Quantity
10740625 tests$80
107407100 tests$180

Product Details


Clone581
ApplicationFlow Cytometry
ReactivityHuman
FormatiF488
Target NameCD34, Gp105-120, My10
IsotypeMouse IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation LaserBlue Laser (488 nm)
RRIDAB_3738779
Research AreasHematopoietic Stem cells, vascular endothelial cells, Adhesion Molecules, Angiogenesis
See All FormatsClone 581

Background Information


CD34 (gp105–120) is a type I monomeric sialomucin-like glycophosphoprotein with a molecular weight of 105–120 kDa, expressed on hematopoietic stem and progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some neural tissues. CD34 serves as a widely used stem cell marker and mediates cell adhesion and lymphocyte homing through interactions with L-selectin and E-selectin ligands. CD34 expression is dynamic and reversible, reflecting specific states of hematopoietic development, including proliferation, differentiation, and altered adhesion properties. Multiple epitopes have been identified based on differential enzymatic sensitivity. Clinically, CD34 is expressed in ~40% of acute myeloid leukemias and 65% of pre-B acute lymphoblastic leukemias, but only 1–5% of acute T-lymphoid leukemias. It is also used to assess stem cell counts for transplantation and can provide prognostic information in certain cancers.

Data Sheets


iF488 Anti-Human CD34 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


Anti-Human CD34 Antibody, Clone 034AB

FITC Anti-Human CD34 Antibody, Clone 034AB

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.