iF488 Human IgG4 isotype control (S228P)

Cat # Size Price Quantity
30300525 ug$150

Product Details


Clone1016AH4M1
ApplicationFlow Cytometry
ReactivityN/A
FormatiF488
Target NameHuman IgG4 isotype control
IsotypeHuman IgG4
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration0.2mg/mL
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageUse at concentrations comparable to those used for the target-specific antibody, iF488 has an excitation max at 491 nm and an emission max at 516 nm.
Excitation LaserBlue Laser (488 nm)
See All FormatsClone 1016AH4M1

Background Information


There are four IgG subclasses (IgG1, 2, 3, and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The measurement of immunoglobulin G can be a diagnostic tool for certain conditions. This clone contains the S228P mutation, which helps minimize the half-antibody formation issue commonly observed in the IgG4 subclass.

Data Sheets


iF488 Human IgG4 isotype control (S228P) TDS

Related Protocols


Flow Cytometry Protocol

Frequently Asked Questions


What should I do if I don't see an isotype control available for my antibody of choice?
Contact Technical Support or request a quote for a custom conjugation of the appropriate isotype and fluorophore.

Why should I use an isotype control?
Isotype controls are antibodies with no known specificity for the target of interest, but matched in host species, isotype, and conjugate to your primary antibody. They are used to measure non-specific binding (e.g., Fc receptor binding, background staining) so that signal from your specific antibody can be distinguished from background noise.

What should I do if my isotype control shows high background binding?
High background is often observed due to a number of causes such as Fc binding, poor cell viability, spillover and unmixing error, as well as monocyte tandem binding. Read this brief tech note to learn about the causes and specific solutions to remove background signals: The Path to True Signals: Removing Background in Flow Cytometry.

Have a product or application question? Consult our FAQs or contact us.