iF488 Rat IgG2b Isotype Control Antibody

Product Details


CloneRTK4530
ApplicationFlow Cytometry
ReactivityN/A
FormatiF488
Target NameRat IgG2b Isotype control
IsotypeRat IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationThis antibody is supplied at a lot-specific concentration.
Storage and HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageUse at concentrations comparable to those used for the target-specific antibody. iF488 has an excitation max at 491 nm and an emission max at 516 nm.
Excitation LaserBlue Laser (488 nm)
RRIDAB_3739129
See All FormatsClone RTK4530

Background Information


The RTK4530 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Data Sheets


iF488 Rat IgG2b Isotype Control Antibody TDS

Related Protocols


Flow Cytometry Protocol

Frequently Asked Questions


What should I do if I don't see an isotype control available for my antibody of choice?
Contact Technical Support or request a quote for a custom conjugation of the appropriate isotype and fluorophore.

Why should I use an isotype control?
Isotype controls are antibodies with no known specificity for the target of interest, but matched in host species, isotype, and conjugate to your primary antibody. They are used to measure non-specific binding (e.g., Fc receptor binding, background staining) so that signal from your specific antibody can be distinguished from background noise.

What should I do if my isotype control shows high background binding?
High background is often observed due to a number of causes such as Fc binding, poor cell viability, spillover and unmixing error, as well as monocyte tandem binding. Read this brief tech note to learn about the causes and specific solutions to remove background signals: The Path to True Signals: Removing Background in Flow Cytometry.

Have a product or application question? Consult our FAQs or contact us.