iF647 Anti-Human CD137 (4-1BB) Antibody

Product Details


Clone4B4-1
ApplicationFlow Cytometry
ReactivityHuman
FormatiF647
Target NameCD137, 4-1BB, ILA, TNFRSF9
IsotypeMouse IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
RRIDAB_3738814
Research AreasActivated T cells, Activated NK cells, Activated Endothelial cells, Cancer Immunology, Autoimmunity, Inflammation, Co-stimulation
See All FormatsClone 4B4-1

Background Information


CD137 is a 39 kDa type I transmembrane protein and a member of the Tumor Necrosis Factor Receptor (TNFR) superfamily. It is expressed on activated T cells, NKT cells, and NK cells, where it functions as a co-stimulatory molecule that promotes T cell proliferation, survival, and monocyte activation through its interaction with 4-1BB ligand. CD137 plays a key role in anti-tumor immunity. Agonistic anti-CD137 antibodies enhance tumor-specific CD8⁺ T cell responses, increase NK and dendritic cell function, and improve antibody-dependent cellular cytotoxicity (ADCC) when used with tumor-targeting monoclonal antibodies. Its expression can be upregulated on NK cells after CD16 ligation, although its effect on NK cell cytotoxicity can vary between human and murine systems. Overall, CD137 is an important immunomodulatory target in cancer immunotherapy.

Isotype Control


iF647 Mouse IgG1 Isotype Control Antibody

Data Sheets


iF647 Anti-Human CD137 (4-1BB) Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


Human 4-1BBL/TNFSF9 Protein (N-Fc)

Human 4-1BBL/TNFSF9 Protein (N-His)

Human CD137/4-1BB/TNFRSF9 Protein (C-His-Avi)

Biotin Human CD137/4-1BB/TNFRSF9 Protein (C-His-Avi)

Human CD137/4-1BB/TNFRSF9 Protein (C-His)

PE Human CD137/4-1BB/TNFRSF9 Protein (C-His)

APC Human CD137/4-1BB/TNFRSF9 Protein (C-His)

Human CD137/4-1BB/TNFRSF9 Protein (C-Fc-Avi)

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.