| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 114503 | 25 tests | $120 | ||
| 114504 | 100 tests | $250 |
| Clone | 282AM2a |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Human |
| Format | iF647 |
| Target Name | CD282, TLR2, Toll like receptor 2, TIL4 |
| Isotype | Mouse IgG2a |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage&Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm. |
| Excitation Laser | Red Laser (633 nm) |
| Isotype Controls | 301511 |
| Antibody Family | Human Antibodies |
| Research Areas | Monocytes, Dendritic cells, Neutrophils, Mast cells, Infectious Diseases, Innate Immunity |
| See All Formats | Clone 282AM2a |
Toll-like receptor 2 (TLR2) is a pattern recognition receptor expressed on innate immune cells such as monocytes, macrophages, dendritic cells, and some epithelial cells. It plays a key role in host defense by recognizing conserved microbial components and initiating inflammatory signaling pathways. Upon activation, TLR2 triggers downstream signaling through adaptor proteins like MyD88, leading to NF-κB activation and production of pro-inflammatory cytokines.
Structurally, TLR2 is a type I transmembrane protein composed of an extracellular domain rich in leucine-rich repeats (LRRs) that mediate ligand recognition, a single transmembrane helix, and an intracellular Toll/IL-1 receptor (TIR) domain responsible for signal transduction. TLR2 typically forms heterodimers with TLR1 or TLR6, which expands its ligand specificity.
TLR2 recognizes a broad range of ligands, including bacterial lipoproteins, peptidoglycan, lipoteichoic acid from Gram-positive bacteria, and certain fungal and viral components. Endogenous ligands released during tissue damage can also activate TLR2, linking it to sterile inflammation.
In disease, dysregulated TLR2 signaling contributes to chronic inflammatory conditions, autoimmune diseases, and sepsis. It also plays roles in cancer by influencing tumor-associated inflammation. Therapeutically, TLR2 is being explored as both a target and a tool, with agonists used as vaccine adjuvants and antagonists investigated to reduce harmful inflammation.
iF647 Anti-human CD282 (TLR2) Antibody TDS
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