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iF647 Anti-Human CD33 Antibody

Cat # Size Price Quantity
11680125 tests$130
116802100 tests$295

  Bulk/Custom

Product Details

CloneLintuzumab
ApplicationFlow Cytometry
ReactivityHuman
FormatiF647
Target NameCD33, Siglec-3, gp67, p67, Siglec3
IsotypeHuman IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
Research AreasMonocytes, Macrophages, Dendritic cells, Innate Immunity, Cancer Marker
See All FormatsClone Lintuzumab

Background Information

Human CD33 is a sialic acid–binding immunoglobulin-like lectin (Siglec-3) expressed primarily on myeloid lineage cells, including monocytes, myeloid progenitors, and some dendritic cells. It functions as an inhibitory receptor that modulates immune cell activation through intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Structurally, CD33 is a type I transmembrane glycoprotein composed of an extracellular domain with Ig-like folds that bind sialylated glycans, a single transmembrane region, and a cytoplasmic tail containing signaling motifs. Its ligands are sialic acid–containing glycoproteins and glycolipids commonly found on cell surfaces, enabling it to regulate cell–cell interactions and dampen immune responses.

CD33 is highly relevant in disease, particularly in acute myeloid leukemia (AML), where it is overexpressed on leukemic blasts, making it a valuable diagnostic marker and therapeutic target. Lintuzumab is a humanized monoclonal antibody directed against CD33 that binds the extracellular domain and promotes immune-mediated cytotoxicity, including antibody-dependent cellular cytotoxicity (ADCC). Although unconjugated lintuzumab has shown modest efficacy, it has been explored in combination therapies and as a targeting moiety for antibody-drug conjugates or radioimmunotherapy. Targeting CD33 allows selective elimination of malignant myeloid cells while sparing non-myeloid tissues, positioning CD33-directed agents, including lintuzumab-based strategies, as important components in AML therapeutic development.

Isotype Control

iF647 Human IgG1 Isotype Control Antibody

Data Sheets

iF647 Anti-Human CD33 Antibody TDS

Related Protocols

Flow Cytometry Protocol

Frequently Asked Questions

How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.
Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.
What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.
Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.