iF647 Anti-Mouse CD11c (integrin αX) Antibody

Product Details


CloneN418-mG1
ApplicationFlow Cytometry
ReactivityMouse
FormatiF647
Target NameCD11c, integrin αX chain, CR4, ITGAX, p150
IsotypeMouse IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation LaserRed Laser (633 nm)
RRIDAB_3739113
Research AreasAdhesion Molecules, Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells, Adaptive Immunity, Autoimmunity, Inflammation
See All FormatsClone N418-mG1

Background Information


CD11c (Integrin αX, CR4, p150) is a ~150 kDa type I transmembrane glycoprotein that pairs non-covalently with β2 integrin (CD18) to form the αXβ2 heterodimer. It is expressed mainly on dendritic cells, with additional expression on NK cells, subsets of intestinal intraepithelial lymphocytes, monocytes/macrophages, granulocytes, and some activated T and B cells. CD11c/CD18 binds iC3b, fibrinogen, and CD54 (ICAM-1), mediating cell–cell adhesion, phagocytosis of opsonized particles, and immune cell trafficking.

Data Sheets


iF647 Anti-Mouse CD11c (integrin αX) Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


Anti-Human CD11b (Integrin αM) Antibody, Clone 011bAB

iF647 Anti-Human CD11b (Integrin αM) Antibody, Clone 011bAB

Anti-Mouse/Human CD11b (Integrin αM) Antibody, Clone M1/70

Biotin Anti-Mouse/Human CD11b (Integrin αM) Antibody, Clone M1/70

Anti-Mouse CD11c (integrin αX) Antibody, Clone N418

FITC Anti-Mouse CD11c (integrin αX) Antibody, Clone N418

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.