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In Vivo Star Anti-Mouse CD115 (CSF1R) Antibody

Cat # Size Price Quantity
5116011 mg$160
5116025 mg$400
51160325 mg$1100

  Bulk/Custom

Product Details

CloneAFS98
ApplicationELISA, WB, Flow cytometry, IHC, ICC, animal model study
Host SpeciesCHO cells
ReactivityMouse
FormatLiquid
Target NameCD115, CSF-1R, CSF1R, M-CSR, c-fms
Product DescriptionIn vivo Grade Recombinant Anti-mouse CD115 Monoclonal Antibody
IsotypeRat IgG2a Kappa
Antibody TypeRecombinant
Regulatory StatusRUO
Purity>95% by reducing SDS-PAGE
Endotoxin< 1 EU per 1 mg of the protein by the LAL method.
Storage Conditions4ºC
GradeIn vivo
Recommended UsageThis product is suitable for in vivo animal use. Optimal amounts need to be determined empirically for each experiment.
Research AreasMonocytes, Macrophages, Dendritic cells, Osteoclasts, MDSCs, Inflammation, Cancer Immunology
See All FormatsClone AFS98

Background Information

CD115, also known as Colony Stimulating Factor 1 Receptor (CSF1R), is a critical cell-surface receptor that plays a fundamental role in regulating the myeloid lineage of immune cells. This receptor controls the production, differentiation, and function of macrophages and monocytes, which are essential components of the innate immune system and serve as the body's first line of defense against pathogens.

CD115 is a transmembrane tyrosine kinase receptor composed of three major structural domains: an extracellular ligand-binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. The receptor is highly expressed on myeloid cells and becomes activated upon ligand binding, triggering intracellular signaling cascades that promote cell survival, proliferation, and differentiation. CD115 can be activated by two distinct ligands: Colony Stimulating Factor 1 (CSF-1) and Interleukin-34 (IL-34). Both cytokines bind to the extracellular domain of CD115, though they may have tissue-specific expression patterns and potentially different biological outcomes.

CD115 has emerged as an important player in various pathological conditions, particularly in cancer. The receptor regulates tumor-associated macrophages (TAMs), which can promote tumor growth, angiogenesis, and immune suppression within the tumor microenvironment. Additionally, CD115 is implicated in inflammatory diseases and conditions involving abnormal macrophage activation, making it a key target for understanding immune dysregulation.

Given its role in disease, CD115 has become an attractive therapeutic target. Anti-CD115 monoclonal antibodies and small molecule CSF1R inhibitors are being developed and tested in clinical trials, particularly for cancer immunotherapy. These therapies aim to deplete or reprogram TAMs, thereby reducing tumor-promoting inflammation and enhancing anti-tumor immune responses. Blocking CD115 signaling represents a promising strategy for modulating the immune microenvironment in cancer and other immune-mediated diseases.

Data Sheets

In Vivo Star Anti-Mouse CD115 (CSF1R) Antibody TDS

Related Protocols

Direct ELISA Protocol

Western Blot Protocol

Flow Cytometry Protocol

Frequently Asked Questions

What are In Vivo Star Biofunctional Antibodies?
In Vivo Star antibodies are premium-grade monoclonal antibodies purified and formulated specifically for use in animal studies. Each lot is manufactured under stringent quality controls to ensure the low endotoxin levels, high purity, and functional activity required for reliable in vivo results.
How are these antibodies different from standard research antibodies (e.g., for Western blot or flow cytometry)?
Standard research antibodies are optimized for in vitro detection assays and may contain preservatives, carrier proteins (like BSA), or higher endotoxin levels unsuitable for injection. In Vivo Star antibodies are formulated in injectable-grade, azide-free, low-endotoxin buffers and validated for functional bioactivity in living systems rather than just antigen binding.
What endotoxin and purity specifications do these antibodies meet?
All In Vivo Star antibodies are tested and certified to meet low-endotoxin thresholds (typically <1 EU/mg, lot-dependent) using the LAL (Limulus Amebocyte Lysate) assay, and are purified to ≥95% purity by SEC-HPLC or SDS-PAGE. Exact specifications are provided on the Certificate of Analysis (CoA) for each lot.
Are these antibodies azide-free and carrier-free?
All In Vivo Star antibodies are formulated without sodium azide and without carrier proteins such as BSA or gelatin, since both can interfere with animal physiology or immune readouts during in vivo studies.
Do you provide isotype controls?
Matched isotype control antibodies (same host species, isotype, and formulation) are available upon request for most clones to support proper experimental controls.
How should In Vivo Star antibodies be stored and handled?
Store at 4°C for short-term use and avoid repeated freeze-thaw cycles, which can reduce bioactivity; aliquoting is recommended for long-term storage per the product-specific storage instructions on the Certificate of Analysis. Always confirm storage conditions and expiration on the CoA that accompanies each lot, as these can vary slightly by antibody.

Have a product or application question? Consult our FAQs or contact us.