Name: In Vivo Star Anti-Mouse CD11c (integrin αX) Antibody
Brand: InnoCyto
SKU: 511801
Price: 160 USD
Availability: InStock

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In Vivo Star Anti-Mouse CD11c (integrin αX) Antibody

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Cat #	Size	Price
511801	1 mg	$160
511802	5 mg	$400
511803	25 mg	$1100

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Product Details

Clone	N418
Application	ELISA, WB, Flow cytometry, IHC, ICC, animal model study
Host Species	CHO cells
Reactivity	Mouse
Format	Liquid
Target Name	CD11c, integrin αX chain, CR4, ITGAX, p150
Product Description	In vivo Grade Recombinant Anti-mouse CD11c Monoclonal Antibody
Isotype	Hamster IgG Kappa
Antibody Type	Recombinant
Regulatory Status	RUO
Purity	>95% by reducing SDS-PAGE
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Storage Conditions	4ºC
Grade	In vivo
Recommended Usage	This product is suitable for in vivo animal use. Optimal amounts need to be determined empirically for each experiment.
RRID	AB_3739392
Research Areas	Adhesion Molecules, Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells, Adaptive Immunity, Autoimmunity, Inflammation
See All Formats	Clone N418

Background Information

CD11c, also known as integrin αX (ITGAX), is a cell surface adhesion and signaling molecule best known as a hallmark marker of dendritic cells in humans and mice. It is also expressed on subsets of monocytes, macrophages, natural killer (NK) cells, and activated T cells. CD11c plays an important role in immune surveillance by regulating cell adhesion, migration, and interactions between antigen-presenting cells and other immune cells.

Structurally, CD11c is a type I transmembrane glycoprotein that heterodimerizes with the integrin β2 subunit (CD18) to form the αXβ2 integrin, also known as complement receptor 4 (CR4). The extracellular domain of CD11c contains an inserted (I) domain, also referred to as an A domain, which is responsible for ligand binding and requires divalent cations such as Mg²⁺ or Mn²⁺ for activity. Like other integrins, CD11c undergoes conformational changes that regulate its affinity for ligands and enable bidirectional signaling. Its short cytoplasmic tail interacts with cytoskeletal and signaling adaptor proteins but lacks intrinsic enzymatic activity.

The primary ligands of CD11c include the complement fragment iC3b, fibrinogen, heparin, and several extracellular matrix proteins. Through binding to iC3b, CD11c contributes to complement-mediated phagocytosis and clearance of opsonized pathogens and apoptotic cells. CD11c also facilitates cell adhesion and migration across endothelial barriers, supporting the trafficking of dendritic cells and monocytes to sites of infection or inflammation and to secondary lymphoid organs.

CD11c is implicated in a range of diseases involving immune dysregulation. In inflammatory and autoimmune disorders, CD11c⁺ myeloid cells contribute to tissue inflammation and antigen presentation, sometimes exacerbating pathology. In cancer, CD11c⁺ dendritic cells play dual roles: they can support antitumor immunity by presenting tumor antigens to T cells, but dysfunctional or tolerogenic CD11c⁺ populations within the tumor microenvironment may promote immune evasion. Altered CD11c expression is also observed in chronic infections, where it can reflect changes in myeloid cell differentiation and function.

Therapeutically, CD11c is most commonly leveraged as a biomarker and targeting handle rather than a direct drug target. Antibodies against CD11c are widely used for the identification, isolation, and depletion of dendritic cells in research and preclinical models. In immunotherapy, CD11c⁺ dendritic cells are central to vaccine strategies, where they are loaded with tumor or pathogen-derived antigens to elicit robust T cell responses. Additionally, targeted delivery of antigens or immunomodulatory agents to CD11c⁺ cells is being explored to enhance vaccine efficacy and modulate immune responses in cancer and autoimmune disease.

Frequently Asked Questions

What are In Vivo Star Biofunctional Antibodies?

In Vivo Star antibodies are premium-grade monoclonal antibodies purified and formulated specifically for use in animal studies. Each lot is manufactured under stringent quality controls to ensure the low endotoxin levels, high purity, and functional activity required for reliable in vivo results.

How are these antibodies different from standard research antibodies (e.g., for Western blot or flow cytometry)?

Standard research antibodies are optimized for in vitro detection assays and may contain preservatives, carrier proteins (like BSA), or higher endotoxin levels unsuitable for injection. In Vivo Star antibodies are formulated in injectable-grade, azide-free, low-endotoxin buffers and validated for functional bioactivity in living systems rather than just antigen binding.

What endotoxin and purity specifications do these antibodies meet?

All In Vivo Star antibodies are tested and certified to meet low-endotoxin thresholds (typically <1 EU/mg, lot-dependent) using the LAL (Limulus Amebocyte Lysate) assay, and are purified to ≥95% purity by SEC-HPLC or SDS-PAGE. Exact specifications are provided on the Certificate of Analysis (CoA) for each lot.

Are these antibodies azide-free and carrier-free?

All In Vivo Star antibodies are formulated without sodium azide and without carrier proteins such as BSA or gelatin, since both can interfere with animal physiology or immune readouts during in vivo studies.

Do you provide isotype controls?

Matched isotype control antibodies (same host species, isotype, and formulation) are available upon request for most clones to support proper experimental controls.

How should In Vivo Star antibodies be stored and handled?

Store at 4°C for short-term use and avoid repeated freeze-thaw cycles, which can reduce bioactivity; aliquoting is recommended for long-term storage per the product-specific storage instructions on the Certificate of Analysis. Always confirm storage conditions and expiration on the CoA that accompanies each lot, as these can vary slightly by antibody.

Have a product or application question? Consult our FAQs or contact us.

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