| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 201813 | 100 μg | $75 | ||
| 201814 | 500 μg | $180 |
| Clone | 2.4G2 |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Mouse |
| Format | Purified |
| Target Name | CD16/32 |
| Isotype | Rat IgG2b |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Protein Concentration | 0.5 mg/mL |
| Storage&Handling | The antibody solution should be stored between 2°C and 8°C |
| Recommended Usage | For flow cytometric analysis, block Fc receptors by pre-incubating cells with a mouse Fc blocker for 5–10 minutes on ice at 1.0 µg per 10⁶ cells in a volume of 100 µL prior to immunostaining. Washing between the blocking step and primary antibody staining is not required. |
| Isotype Controls | N/A |
| Antibody Family | Mouse Antibodies |
| See All Formats | Clone 2.4G2 |
CD16/32 are Fc-gamma receptors (FcγRs) expressed on a variety of immune cells, including B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD16 corresponds to the low-affinity Fc receptor III (FcγRIII), while CD32 corresponds to Fc receptor II (FcγRII). These receptors bind antibody-antigen immune complexes, linking innate and adaptive immunity and mediating adaptive immune responses. In research, antibodies against CD16/CD32 are commonly used to block Fc receptor-mediated interactions, preventing non-specific binding of antibodies or immunoglobulin complexes to immune cells during experiments such as flow cytometry and immunohistochemistry, thereby improving experimental accuracy.
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