PE Anti-mouse CD19 Antibody

Cat # Size Price Quantity
20190725 tests$70
201908100 tests$180

Product Details


Clone1D3
ApplicationFlow Cytometry
ReactivityMouse
FormatPE
Target NameCD19, B4, CVID3, Leu-12, MGC12802
IsotypeRat IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation LaserBlue Laser (488 nm) Green/Yellow laser (532/561nm)
RRIDAB_3739072
Research AreasB cells, Plasma cells, Cancer Marker, follicular Dendritic cells
See All FormatsClone 1D3

Background Information


CD19, also known as B-lymphocyte antigen CD19, B4, Leu-12, or CVID3, is a type I transmembrane glycoprotein encoded by the CD19 gene. It is expressed throughout B cell development from the pro-B stage to mature B cells and is present on most B lineage cells. CD19 functions as an adaptor protein, recruiting cytoplasmic signaling molecules to the membrane, and forms part of the CD19/CD21/CD81 complex, which lowers the activation threshold for B cell receptor (BCR) signaling. Because of its consistent expression on B cells, CD19 serves as a key biomarker for B cell development and is widely used in the diagnosis of B cell lymphomas and leukemias. It is also a major therapeutic target in immunotherapies, including CAR T-cell therapy for B cell malignancies.

Data Sheets


PE Anti-mouse CD19 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


In Vivo Star Anti-Mouse CD20 Antibody, Clone 18B12

In Vivo Star Anti-Mouse CD19 Antibody, Clone 1D3

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.