PE Rat IgM Isotype Control Antibody

Cat # Size Price Quantity
30270325 ug$75

Product Details


CloneRTK2118
ApplicationFlow Cytometry
ReactivityN/A
FormatPE
Target NameRat IgM isotype control
IsotypeRat IgM
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration0.2 mg/mL
Storage&HandlingStore the antibody undiluted at 2°C to 8°C.
Recommended UsageUse at concentrations comparable to those used for the target-specific antibody. PE has an excitation max at 565 nm and an emission max at 575 nm.
Excitation LaserBlue Laser (488 nm) Green/Yellow laser (532/561nm)
RRIDAB_3739232
See All FormatsClone RTK2118

Background Information


Immunoglobulin M (IgM) is the largest antibody isotype and the first produced during an initial immune response. Structurally, it forms a pentamer of five immunoglobulin units, giving it a high molecular weight of about 900 kDa and up to ten antigen-binding sites, though not all can engage simultaneously. Because of its size, IgM remains primarily in the serum and is produced largely in the spleen. Rat IgM functions similarly, serving as an early, broad-acting antibody produced by rat B cells to recognize and neutralize pathogens. It is widely used in research—as a control reagent, in diagnostic assays, and in studies of infection and immunity. Various established laboratory methods exist for producing and purifying rat IgM, making it a valuable tool for advancing immunological understanding and supporting the development of new disease treatments

Data Sheets


PE Rat IgM Isotype Control Antibody TDS

Related Protocols


Flow Cytometry Protocol

Frequently Asked Questions


What should I do if I don't see an isotype control available for my antibody of choice?
Contact Technical Support or request a quote for a custom conjugation of the appropriate isotype and fluorophore.

Why should I use an isotype control?
Isotype controls are antibodies with no known specificity for the target of interest, but matched in host species, isotype, and conjugate to your primary antibody. They are used to measure non-specific binding (e.g., Fc receptor binding, background staining) so that signal from your specific antibody can be distinguished from background noise.

What should I do if my isotype control shows high background binding?
High background is often observed due to a number of causes such as Fc binding, poor cell viability, spillover and unmixing error, as well as monocyte tandem binding. Read this brief tech note to learn about the causes and specific solutions to remove background signals: The Path to True Signals: Removing Background in Flow Cytometry.

Have a product or application question? Consult our FAQs or contact us.