Name: SARS-CoV-2 Spike Trimer Protein (C-His)
Brand: InnoCyto
SKU: 603701
Price: 195 USD
Availability: InStock

Shop Now Contact

SARS-CoV-2 Spike Trimer Protein (C-His)

SARS-CoV2 Spike Trimer Protein with C-His tag on SDS-PAGE under reducing conditions. The gel was stained for 1 hour with BlinkBlue Protein Staining Buffer (Catalog 700102). The purity of this protein appears to be greater than 90%.

Recombinant SARS-CoV2 Spike Trimer protein is coated at 2ug/mL (200ng/well). Biotinylated Human ACE2 Protein (N-His, catalog # 600003) can bind SARS-CoV2 Spike Trimer in the dose-dependent manner (Quality tested).

Recombinant SARS-CoV2 Spike Trimer protein is coated at 2ug/mL (200ng/well). Anti-SARS-CoV2 spike antibody (clone 5D9 from Novoproteins) can bind SARS-CoV2 Spike Trimer in the dose-dependent manner (Quality tested).

Cat #	Size	Price	Quantity
603701	25 ug	$195
603702	100 ug	$395

Bulk/Custom

Product Details

Application	ELISA, BLI
Format	Liquid, Purified
Expression Host	HEK293
Target Name	SARS-CoV2 Spike Protein Trimer, Spike trimer
Species	SARS-CoV-2
Accession Number	QHD43416.1
Sources	Recombinant SARS-CoV-2 Spike protein (Val16-Gln1208 ) with furin mutation, and C-terminus trimer motif and His tag was expressed in 293 Cells.
Molecular Weight	This protein has a predicted molecular weight of 138 kDa. Under DTT-reducing conditions, the protein migrates at approximately 150-180 kDa on SDS-PAGE.
Affinity Tag	C-His
Purity	>90% based on SDS-PAGE under reducing condition
Regulatory Status	RUO
Formulation	1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level	Not tested
Protein Concentration	25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling	Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Research Areas	SARS-CoV-2, Infectious Diseases

Background Information

The spike protein forms a homotrimer on the viral surface, with each monomer composed of two functional subunits: S1 and S2. The spike protein is heavily glycosylated, shielding it from host antibodies and contributing to immune evasion. Spike trimer protein mediates the membrane fusion and viral entry. Upon ACE2 engagement and proteolytic cleavage (e.g., by furin or TMPRSS2), the S1 subunit dissociates, and S2 undergoes a conformational change to trigger membrane fusion. The trimeric spike is the main target for neutralizing antibodies and vaccines, making its structural integrity and mutations within it (especially in RBD and S1/S2 cleavage site) critical to viral infectivity, immune escape, and vaccine efficacy.

Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?

The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.

What types of tagged proteins are best for my application?

Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.

Does the tag affect the protein's biological activity or binding function?

Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.

Search Tips