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Anti-Human EGFR (Nimotuzumab Biosimilar)

Cat # Size Price Quantity
5041011 mg$450
5041025 mg$1200
50410320 mg$3900

  Bulk/Custom

Product Details

CloneNimotuzumab
ApplicationFlow cytometry, animal model study
Host SpeciesMammalian cells
ReactivityHuman
FormatLiquid
Product DescriptionAnti-Human EGFR (Nimotuzumab Biosimilar)
IsotypeHuman IgG4
Regulatory StatusRUO
ClonalityRecombinant
ImmunogenHuman EGFR / ErbB-1
Species specificityHuman
Purity>95% by reducing SDS-PAGE
GradeIn vivo
Storage Conditions4ºC
Maximal Shelf Life12 months
SynonymsErbB1, HER1
Target NameEGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
Antibody TypeRecombinant
Research AreasCancer Marker, Epithelial cells
See All FormatsClone Nimotuzumab

Background Information

Nimotuzumab is a humanized monoclonal antibody of the immunoglobulin G1 (IgG1) subclass, engineered to selectively target the extracellular domain of the epidermal growth factor receptor (EGFR), also known as ErbB1 or HER1. Structurally, Nimotuzumab is composed of two identical heavy chains and two identical light chains interconnected by disulfide bonds, forming the characteristic Y-shaped quaternary structure of immunoglobulins. The molecule has an approximate molecular weight of 150 kilodaltons (kDa) and a glycoprotein composition typical of monoclonal antibodies expressed in mammalian systems, such as Chinese Hamster Ovary (CHO) cells, which ensure accurate folding and N-linked glycosylation.

The variable domains of the heavy (VH) and light (VL) chains contain complementarity-determining regions (CDRs) responsible for antigen recognition. Derived from murine antibody sequences and grafted onto human immunoglobulin frameworks, these CDRs define Nimotuzumab’s high-affinity, selective binding to subdomain III of the EGFR’s extracellular region. This domain is essential for the receptor’s interaction with natural ligands, such as epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-α). By binding to this epitope, Nimotuzumab competitively inhibits ligand-induced receptor dimerization and autophosphorylation, thereby blocking downstream activation of EGFR-dependent intracellular signaling cascades, including the RAS-RAF-MEK-ERK and PI3K-AKT-mTOR pathways that regulate cell survival, proliferation, and differentiation.

The Fc (fragment crystallizable) region of Nimotuzumab, typical of the IgG1 isotype, provides structural stability and prolongs serum half-life through interaction with the neonatal Fc receptor (FcRn). It also allows for secondary effector functions such as antibody-dependent cellular cytotoxicity (ADCC) and complement fixation under experimental conditions. Overall, Nimotuzumab exemplifies rational antibody engineering - integrating the ligand-blocking capabilities of an EGFR antagonist with the pharmacokinetic and effector properties of a stable IgG1 framework.

Data Sheets

Anti-Human EGFR (Nimotuzumab Biosimilar) TDS

Related Protocols

Flow Cytometry Protocol

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Frequently Asked Questions

What are InnoCyto Biosimilar products?
InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.
How similar are these biosimilars to the original therapeutic antibody?
Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.
Are these antibodies suitable for in vivo studies?
Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.
What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?
A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.
What quality and purity standards do biosimilar antibodies meet?
All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.
Which therapeutic targets and mechanisms are covered in the Biosimilar line?
The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

Have a product or application question? Consult our FAQs or contact us.