Shop Now Contact

Anti-Human PD-L1 (Envafolimab Biosimilar)

Cat # Size Price Quantity
5025011 mg$250
5025025 mg$650
50250320 mg$2000

  Bulk/Custom

Product Details

CloneEnvafolimab
ApplicationFlow cytometry, animal model study
Host SpeciesMammalian cells
ReactivityHuman
FormatLiquid
Product DescriptionAnti-Human PD-L1 (Envafolimab Biosimilar)
IsotypeHuman IgG1
Regulatory StatusRUO
ClonalityRecombinant
ImmunogenHuman PD-L1
Species specificityHuman
Purity>95% by reducing SDS-PAGE
GradeIn vivo
Storage Conditions4ºC
Maximal Shelf Life12 months
SynonymsCD274, B7-H1
RRIDAB_3739301
Target NamePD-L1, PDL1 CD274, B7-H1
Antibody TypeRecombinant
Research AreasActivated T cells, Activated B cells, Monocytes, Macrophages, Dendritic cells, NK cells, Immune Checkpoints, Cancer Immunology
See All FormatsClone Envafolimab

Background Information

Envafolimab, also known as KN035, is a novel recombinant single-domain antibody–Fc fusion protein engineered to specifically target programmed death-ligand 1 (PD-L1). Structurally, Envafolimab is distinct from conventional monoclonal antibodies because it consists of only the heavy-chain variable domain (VH) derived from camelid antibody fragments, fused directly to the Fc (fragment crystallizable) region of human immunoglobulin G1 (IgG1). This configuration results in a smaller, single-chain architecture with a molecular weight of approximately 80 kilodaltons (kDa), roughly half that of a full-length IgG molecule. The molecule is produced in mammalian expression systems to maintain proper folding, disulfide bond formation, and glycosylation within the Fc portion.

The single-domain variable region of Envafolimab provides specificity for PD-L1 through high-affinity interactions dominated by hydrogen bonding and hydrophobic contacts. Upon binding, Envafolimab effectively blocks PD-L1 from engaging its receptors, programmed death-1 (PD-1) and B7.1 (CD80), on activated T lymphocytes and antigen-presenting cells. This blockade disrupts inhibitory signaling pathways that would otherwise reduce T-cell proliferation and cytokine secretion. Functionally, this mechanism restores or enhances immune cell activation in experimental systems, making Envafolimab a valuable tool for the study of immune checkpoint regulation and ligand–receptor signaling.

The Fc region of Envafolimab contributes structural stability, mediates dimerization, and extends circulatory half-life through interactions with neonatal Fc receptors (FcRn), which protect the molecule from lysosomal degradation. Because the Fc segment is derived from IgG1, it retains potential for limited Fc receptor binding, though it is not primarily designed for effector function. The compact and stable single-domain architecture grants Envafolimab notable advantages in solubility, tissue penetration, and molecular stability. Overall, Envafolimab exemplifies advanced protein engineering that fuses minimal antigen-binding domains with antibody Fc functionality to create a small, high-affinity immune checkpoint inhibitor optimized for mechanistic and biochemical research applications.

Data Sheets

Anti-Human PD-L1 (Envafolimab Biosimilar) TDS

Related Protocols

Flow Cytometry Protocol

Related Products

Anti-Human CD80 (Galiximab Biosimilar), Clone Galiximab

Human B7-1 (CD80) Protein (C-His-Avi)

Biotin Human B7-1 (CD80) Protein (C-His-Avi)

Human B7-1 (CD80) Protein (C-His)

Anti-Human PD-L1 (Durvalumab Biosimilar), Clone Durvalumab

Frequently Asked Questions

What are InnoCyto Biosimilar products?
InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.
How similar are these biosimilars to the original therapeutic antibody?
Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.
Are these antibodies suitable for in vivo studies?
Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.
What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?
A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.
What quality and purity standards do biosimilar antibodies meet?
All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.
Which therapeutic targets and mechanisms are covered in the Biosimilar line?
The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

Have a product or application question? Consult our FAQs or contact us.