Name: Anti-Human PD1 (Pembrolizumab Biosimilar)
Brand: InnoCyto
SKU: 504701
Price: 190 USD
Availability: InStock

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Anti-Human PD1 (Pembrolizumab Biosimilar)

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Cat #	Size	Price
504701	1 mg	$190
504702	5 mg	$590
504703	20 mg	$1100

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Product Details

Clone	Pembrolizumab
Application	Flow cytometry, animal model study
Host Species	Mammalian cells
Reactivity	Human
Format	Liquid
Product Description	Anti-Human PD-1 (Pembrolizumab Biosimilar)
Isotype	Human IgG4
Regulatory Status	RUO
Clonality	Recombinant
Immunogen	Human PD1
Species specificity	Human
Purity	>95% by reducing SDS-PAGE
Grade	In vivo
Storage Conditions	4ºC
Maximal Shelf Life	12 months
Synonyms	CD279
RRID	AB_3739323
Target Name	PD1, PD-1, PDCD1, CD279, SLEB2
Antibody Type	Recombinant
Research Areas	Immune Checkpoints, Activated T cells, Cancer Immunology, Tregs, Tfh cells, Activated B cells
See All Formats	Clone Pembrolizumab

Background Information

Pembrolizumab is a fully humanized monoclonal antibody of the immunoglobulin G4 kappa (IgG4κ) subclass engineered to specifically bind to the programmed death-1 (PD-1, also known as CD279) receptor expressed on activated T cells. Structurally, Pembrolizumab is a glycoprotein with a molecular weight of approximately 149 kilodaltons (kDa). The molecule consists of two identical heavy chains and two identical light chains joined by disulfide bonds, forming the characteristic Y-shaped architecture typical of immunoglobulins. Each heavy chain is composed of one variable (VH) and three constant (CH1-CH3) domains, while each light chain contains one variable (VL) and one constant (CL) domain. It is produced in mammalian expression systems, such as Chinese Hamster Ovary (CHO) cells, to ensure correct folding, glycosylation, and molecular stability.

The variable domains of Pembrolizumab contain complementarity-determining regions (CDRs) that form the antigen-binding sites responsible for recognizing a specific epitope on the PD-1 receptor. These CDRs establish high-affinity, non-covalent interactions with the extracellular domain of PD-1. By occupying the ligand-binding region of PD-1, Pembrolizumab effectively blocks its interaction with programmed death-ligand 1 (PD-L1) and programmed death-ligand 2 (PD-L2). This blockade inhibits the intracellular signaling cascade initiated by PD-1 engagement, which normally leads to suppression of T-cell activation, cytokine production, and proliferation. In experimental systems, this results in enhanced T-cell signaling and immune response regulation.

The Fc (fragment crystallizable) region of Pembrolizumab is derived from the IgG4 isotype and includes an engineered S228P mutation in the hinge region to improve structural integrity by preventing half-antibody exchange. The IgG4 Fc configuration minimizes binding to Fc gamma receptors (FcγRs) and complement component C1q, thus greatly reducing antibody-dependent and complement-mediated cytotoxicity. Overall, Pembrolizumab exemplifies advanced antibody engineering, combining high receptor specificity, precise inhibitory activity, and optimized molecular stability for studies of immune checkpoint regulation and T-cell receptor–ligand dynamics.

Frequently Asked Questions

What are InnoCyto Biosimilar products?

InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.

How similar are these biosimilars to the original therapeutic antibody?

Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.

Are these antibodies suitable for in vivo studies?

Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.

What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?

A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.

What quality and purity standards do biosimilar antibodies meet?

All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.

Which therapeutic targets and mechanisms are covered in the Biosimilar line?

The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

Have a product or application question? Consult our FAQs or contact us.

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