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Anti-Human VEGF (Aflibercept Biosimilar)

Cat # Size Price Quantity
5004011 mg$500
5004025 mg$1200
50040320 mg$3900

  Bulk/Custom

Product Details

CloneAflibercept
ApplicationNeutralization, Intracellular Flow cytometry, animal model study
Host SpeciesMammalian cells
ReactivityHuman
FormatLiquid
Product DescriptionAnti-Human VEGF (Aflibercept Biosimilar)
IsotypeHuman IgG1-Fc
Regulatory StatusRUO
ClonalityRecombinant
Species specificityHuman
Purity>95% by reducing SDS-PAGE
GradeIn vivo
Min Sample Size1 mg
Storage Conditions4ºC
Maximal Shelf Life12 months
SynonymsVEGFR, VEGFR1, VEGFR2
Target NameVEGF
Research AreasCytokines, Angiogenesis, Hypoxia
See All FormatsClone Aflibercept

Background Information

Aflibercept is a recombinant fusion protein specifically engineered to act as a decoy receptor for vascular endothelial growth factors (VEGFs). Structurally, the molecule combines the ligand-binding domains of two distinct human receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR), fused to the Fc portion of human immunoglobulin G1 (IgG1). More precisely, Aflibercept contains the extracellular domain 2 of VEGFR-1 and domain 3 of VEGFR-2, which together form a high-affinity binding site for VEGF ligands. The resultant fusion protein has a molecular mass of approximately 115 kilodaltons (kDa) and is produced in mammalian expression systems such as Chinese Hamster Ovary (CHO) cells to ensure proper folding, disulfide bonding, and glycosylation.

The Fc region of Aflibercept serves several critical structural and functional purposes. It provides molecular stability, confers dimerization through disulfide bridges to form a Y-shaped homodimer, and extends the molecule's circulatory half-life via interactions with neonatal Fc receptors (FcRn). The fusion of the VEGF-binding domains to this Fc backbone effectively produces a hybrid receptor that retains the natural ligand-binding specificity of VEGFR molecules while gaining the pharmacokinetic benefits typical of antibodies. Each Aflibercept dimer can bind multiple molecules of VEGF, acting as a soluble trap with high binding affinity in the picomolar range.

Functionally, Aflibercept acts as a potent VEGF antagonist by sequestering VEGF-A, VEGF-B, and placental growth factor (PlGF) before these ligands can interact with their receptors on endothelial cells. By intercepting VEGF signaling, the molecule effectively prevents receptor dimerization and downstream activation of signaling pathways such as MAPK and PI3K-AKT, which are involved in endothelial cell proliferation, survival, and permeability. This molecular sequestration mechanism makes Aflibercept an important biological model for studying angiogenesis regulation, receptor-ligand affinity optimization, and engineered protein-receptor interactions in molecular biology and biochemistry.

Data Sheets

Anti-Human VEGF (Aflibercept Biosimilar) TDS

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Frequently Asked Questions

What are InnoCyto Biosimilar products?
InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.
How similar are these biosimilars to the original therapeutic antibody?
Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.
Are these antibodies suitable for in vivo studies?
Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.
What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?
A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.
What quality and purity standards do biosimilar antibodies meet?
All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.
Which therapeutic targets and mechanisms are covered in the Biosimilar line?
The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

Have a product or application question? Consult our FAQs or contact us.