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HexA-His/HexB-FLAG Heterodimer Protein

Cat # Size Price Quantity
60100125 ug$245
601002100 ug$595

  Bulk/Custom

Product Details

ApplicationELISA
FormatLiquid, Purified
Expression HostCHO
Target NameHexosaminidase A, Hexosaminidase B
SpeciesHuman
Accession NumberP07686/P06865
SourcesRecombinant human HexB (Ala43-Met556) with C-terminus DYKDDDDK tag and human HexA (Leu23-Thr529) with C-terminus His tag are co-expressed in CHO cells. Heterodimer protein is purified sequentially by anti-FLAG tag resin and Nickel resin.
Molecular WeightHuman HexA protein has the predicted molecular weight of 60 kD and human HexB protein has the predicted molecular weight of 59.8 kD . Under DTT-reducing conditions, both proteins migrate at approximately 65 kDa on SDS-PAGE.
Affinity TagC-His and C-DYKDDDDK
Purity>95% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation25 mM Tris, 150 mM NaCl, pH 7.5 (0.2 μm filtered)
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered 25 mM Tris, 150 mM NaCl, pH 7.5. For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Research AreasMetabolism

Background Information

Beta-hexosaminidases are lysosomal enzymes that hydrolyze terminal N-acetyl-D-hexosamine residues from GM2 gangliosides and globo-sphingolipids. They exist in three isoforms: Hex A (αβ), Hex B (ββ), and Hex S (αα), formed by different combinations of α and β subunits encoded by the HEXA and HEXB genes. Recombinant HexA-His/HexB-FLAG Heterodimer corresponds to Hex A isoform.

Data Sheets

HexA-His/HexB-FLAG Heterodimer Protein TDS

Related Protocols

Direct ELISA Protocol

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Human HexA Protein (C-His, CHO Expressed)

Human HexA Protein (C-FLAG)

Human HexB Protein (C-FLAG)

Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?
The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.
What types of tagged proteins are best for my application?
Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.
Does the tag affect the protein's biological activity or binding function?
Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.