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Human PPT1 Protein (C-FLAG)

Cat # Size Price Quantity
60130125 ug$195
601302100 ug$395

  Bulk/Custom

Product Details

ApplicationELISA, BLI
FormatLiquid, Purified
Expression HostCHO
Target NamePalmitoyl-protein thioesterase 1
SpeciesHuman
Accession NumberP50897.1
Sourceshuman PPT1 (Asp28-Gly306) with C-terminus DYKDDDDK tag was expressed in CHO cells
Molecular WeightThis protein has a predicted molecular weight of 32.6 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35-40 kDa on SDS-PAGE.
Affinity TagC-DYKDDDDK
Purity>95% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation1xPBS buffer, pH6.5, 0.22 µm filtered
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Research AreasMetabolism

Background Information

Palmitoyl-protein thioesterase 1 (PPT1) is a lysosomal enzyme that hydrolyzes thioester-linked palmitate from S-palmitoylated proteins, facilitating their degradation during lysosomal processing. PPT1 is a glycosylated monomer with a catalytic triad and fatty acid-binding groove, and plays a key role in the autophagy-lysosome pathway. Mutations in the PPT1 gene cause infantile neuronal ceroid lipofuscinosis (INCL), a severe lysosomal storage disorder characterized by accumulation of lipid-modified proteins, leading to neurodegeneration, mental retardation, and early death. In addition to INCL, impaired PPT1 function has been associated with Huntington’s disease, Alzheimer’s disease, schizophrenia, and cancer, due to its influence on synaptic function and protein trafficking.

Data Sheets

Human PPT1 Protein (C-FLAG) TDS

Related Protocols

Direct ELISA Protocol

Related Products

Human PPT1 Protein (C-His)

Frequently Asked Questions

Why are Recombinant Proteins expressed with epitope tags?
The majority of InnoCyto's recombinantly expressed proteins are engineered with a short, well-characterized peptide sequence (e.g., His-tag, Fc-tag, Flag-tag, or similar) fused to the protein of interest. The tag enables reliable detection, purification, or immobilization of the protein using commercially available anti-tag antibodies or affinity resins, without needing a custom antibody against the native protein.
What types of tagged proteins are best for my application?
Common tag options include His-tag (for affinity purification via Ni-NTA/IMAC and detection via anti-His antibodies), Fc-tag (for use in binding assays, ELISA capture, and dimerization studies), and Flag-tag (for high-specificity detection and immunoprecipitation). Tag choice should be based on your intended workflow — purification-focused work often favors His-tag, while functional binding or capture-based assays often benefit from Fc-tag.
Does the tag affect the protein's biological activity or binding function?
Tag placement (N-terminal vs. C-terminal) and type are selected during design to minimize interference with the protein's native folding and functional binding site, and each tagged protein is validated for activity in relevant binding or functional assays. Product pages indicate the tag location and confirm the functional validation performed for that specific protein.

Have a product or application question? Consult our FAQs or contact us.