iF488 Anti-Human CD279 (PD1) Antibody

Cat # Size Price Quantity
10140925 tests$80
101410100 tests$220

Product Details


Clone279AM1
ApplicationFlow Cytometry
ReactivityHuman
FormatiF488
Target NameCD279, PD1, PD-1
IsotypeMouse IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation LaserBlue Laser (488 nm)
RRIDAB_3738613
Research AreasImmune Checkpoints, Activated T cells, Cancer Immunology, Tregs, Tfh cells, Activated B cells
See All FormatsClone 279AM1

Background Information


CD279, also known as Programmed cell death 1 (PD-1), cell surface receptor which binds to PD-L1 and PD-L2 ligands; functions as immune checkpoint with important role in down regulating immune system by preventing T-cell activation. Targeted by Nivolumab, Pembrolizumab and other monoclonal antibodies in a new class of cancer drugs. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Data Sheets


iF488 Anti-Human CD279 (PD1) Antibody TDS

Related Protocols


Flow Cytometry Protocol

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Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.