Name: Anti-Human PD1 (Nivolumab Biosimilar)
Brand: InnoCyto
SKU: 504203
Price: 1100 USD
Availability: InStock

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Anti-Human PD1 (Nivolumab Biosimilar)

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Cat #	Size	Price
504201	1 mg	$190
504202	5 mg	$590
504203	20 mg	$1100

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Product Details

Clone	Nivolumab
Application	Flow cytometry, animal model study
Host Species	Mammalian cells
Reactivity	Human
Format	Liquid
Product Description	Anti-Human PD-1 (Nivolumab Biosimilar)
Isotype	Human IgG4
Regulatory Status	RUO
Clonality	Recombinant
Immunogen	Recombinant human PD-1-Fc protein
Clone Number	5C4.B8
Species specificity	Human
Purity	>95% by reducing SDS-PAGE
Grade	In vivo
Storage Conditions	4ºC
Maximal Shelf Life	12 months
Synonyms	CD279
Target Name	PD1, PD-1, PDCD1, CD279, SLEB2
Antibody Type	Recombinant
Research Areas	Immune Checkpoints, Activated T cells, Cancer Immunology, Tregs, Tfh cells, Activated B cells
See All Formats	Clone Nivolumab

Background Information

Nivolumab is a fully human immunoglobulin G4 kappa (IgG4κ) monoclonal antibody engineered to target programmed death-1 (PD-1), an immune checkpoint receptor expressed on activated T cells. Structurally, it has a molecular weight of approximately 146 kilodaltons (kDa) and comprises two identical heavy chains and two identical light chains connected by interchain disulfide bonds, forming the canonical Y-shaped structure of IgG molecules. The antibody is produced in mammalian expression systems, such as Chinese Hamster Ovary (CHO) cells, ensuring correct folding, assembly, and glycosylation that conform to native human immunoglobulin characteristics. To improve structural integrity, the IgG4 Fc region incorporates a stabilizing S228P hinge mutation that prevents half-antibody exchange, a property commonly optimized in therapeutic IgG4 frameworks.

The variable regions of Nivolumab’s heavy (VH) and light (VL) chains contain complementarity-determining regions (CDRs) that form the high-affinity antigen-binding site. These CDRs mediate specific recognition of an extracellular epitope on the PD-1 receptor through a combination of hydrogen bonding and hydrophobic interactions, exhibiting binding affinities in the nanomolar range. By occupying this epitope, Nivolumab blocks the interaction between PD-1 and its ligands, programmed death-ligand 1 (PD-L1) and programmed death-ligand 2 (PD-L2), effectively interrupting the inhibitory signaling cascade that downregulates T-cell activation. In experimental systems, this blockade restores intracellular signaling involving ZAP-70 and PI3K, enhancing T-cell receptor–mediated activation and cytokine production.

The Fc (fragment crystallizable) region of Nivolumab confers structural stability, extended serum half-life, and reduced immune effector activity. The IgG4 isotype displays low affinity for Fc gamma receptors (FcγRs) and complement component C1q, minimizing antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Interaction with the neonatal Fc receptor (FcRn) contributes to molecular recycling and sustained systemic persistence.

Frequently Asked Questions

What are InnoCyto Biosimilar products?

InnoCyto biosimilars are research-grade recombinant antibodies or proteins engineered to closely replicate the sequence, structure, and target-binding activity of approved or well-characterized therapeutic antibodies (e.g., checkpoint inhibitors, anti-cytokine biologics). They are intended for research use to study the mechanism of action, efficacy, and combination potential of these therapeutic classes without the cost or access restrictions of clinical-grade material.

How similar are these biosimilars to the original therapeutic antibody?

Each biosimilar is produced using the published or inferred variable region sequence of the reference therapeutic and is expressed recombinantly to match the parent antibody's target specificity, isotype, and general binding profile. While designed for high functional similarity, InnoCyto biosimilars are for research use only and are not clinically validated equivalents of the approved drug product.

Are these antibodies suitable for in vivo studies?

Yes. InnoCyto biosimilars are manufactured with the same low-endotoxin, azide-free, carrier-free standards used across our in vivo product lines, making them suitable for animal efficacy, combination, and mechanism-of-action studies. Product pages indicate whether a given biosimilar is validated for in vivo use, in vitro use, or both.

What is the difference between a biosimilar and a recombinant "research antibody" targeting the same protein?

A biosimilar is specifically designed to mimic a known, named therapeutic antibody (matching its clinical target epitope and mechanism), whereas a general recombinant research antibody may target the same protein but bind a different epitope or lack the same functional properties. Biosimilars are the right choice when your study is benchmarking against, or modeling the action of, an approved or clinically studied biologic.

What quality and purity standards do biosimilar antibodies meet?

All biosimilars undergo the same rigorous QC pipeline as our other antibody lines: ≥95% purity by SDS-PAGE/SEC-HPLC, low-endotoxin testing via LAL assay, and binding/functional validation against the intended target. Certificates of Analysis (CoA) are provided per lot with full specifications.

Which therapeutic targets and mechanisms are covered in the Biosimilar line?

The line spans major immuno-oncology and immunology targets, including immune checkpoint pathways (e.g., PD-1/PD-L1, CTLA-4), cytokine and cytokine-receptor blockers, and other clinically relevant mechanisms. See each product page for the specific reference therapeutic and mechanism of action represented.

Have a product or application question? Consult our FAQs or contact us.

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